From KO to KI: The New Era of CRISPR Gene Editing in Research
Introduction For many scientists, CRISPR knockout (KO) experiments have become routine. Silencing a gene, checking the phenotype, and drawing conclusions about its role is often straightforward. But as projects advance, researchers quickly realize that “turning off” a gene doesn’t always give the answers they need. Modeling patient-specific mutations, tagging proteins for live-cell tracking, or testing…

